Reproducibility of Differential Centrifugation Experiments in Tissue Fractionation

Fractionation of Mammalian Liver Cells by Differential Centrifugation

1. A method is described whereby the major components of liver suspensions are segregated according to size into three main fractions: (a) a large granule fraction composed of elements approximately 0.5 to 2 micro in diameter; (b) a microsome fraction composed of submicroscopic elements approximately 80 to 150 mmicro in diameter; and, (c) a supernate fraction containing the soluble components o...

optimization of the fractionation of hepg2 cell line by differential centrifugation

Subcellular fractionation by differential and zonal centrifugation of aerobically grown glucose-de-repressed Saccharomyces carlsbergensis.

1. Homogenates were prepared from sphaeroplasts of aerobically grown glucose-de-repressed Saccharomyces carlsbergensis and the distributions of marker enzymes were investigated after differential centrifugation. Cytochrome c oxidase and cytochrome c were sedimented almost completely at 10(5)g-min, and this fraction also contained 37% of the catalase, 27% of the acid p-nitrophenyl phosphatase, 5...

A method for rapid fractionation of particulate systems by gradient differential centrifugation.

IPIT initial experiments on the fractionation of mitochondria into subparticulate constituents, it became apparent that for isolation of the particles, a method more satisfactory than the customary methods of differential centrifugation was needed. The subject of this report is a simple and rapid method for obtaining relatively uncontaminated preparations of particulate cell components by gradi...

Calf Thymus Composition. A Comparison of Differential Centrifugation and Chemical Fractionation

The extraction behavior of thymus and the composition of fractions prepared from this organ has been studied. Sequential extraction methods using 0.15 NaC1 followed by water gave information with respect to the weight fraction of cytoplasmic and nuclear constituents. Lipide, nucleic acid, and electrophoretic analysis of the extracts provided additional information. A less complex electrophoreti...